Experiment 2: Diffusion Concentration Gradients and Membrane Permeability Data Tables Table 2: Indicator Reagent Data Indicator…
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Question “Experiment 2: Diffusion Concentration Gradients and Membrane Permeability Data Tables Table 2: Indicator Reagent Data Indicator…”
Experiment 2: Diffusion Concentration Gradients and Membrane
Permeability
Data Tables
Table 2: Indicator Reagent Data
Indicator | Starch Positive | Starch Negative | Glucose Positive | Glucose Negative |
Glucose Test Strip | n/a | n/a | green | yellow |
IKI Solution | dark blue | light brown | n/a | n/a |
Table 3: Diffusion of Starch and Glucose Over
Time
Indicator | Dialysis Bag After 60 Minutes | Beaker Water After 60 Minutes |
Glucose Test Strip | yellow | green ( if all glucose passed to the beaker from the bag) |
IKI | from incolore changed to blue dark color | pale yellow ( as iodine concentration diminishes in the beaker |
Post-Lab Questions
- State your hypothesis.
- Why is it necessary to have positive and negative controls in
this experiment? - Draw a diagram of the experimental set-up. Use arrows to depict
the movement of each substance in the dialysis bag and the
beaker. - Which substance(s) crossed the dialysis membrane? Support your
response with data-based evidence. - Which molecules remained inside of the dialysis bag?
- Did all of the molecules diffuse out of the bag into the
beaker? Why or why not?
Reflection (Discuss what you have learned by
doing this experiment. How have your ideas changed? Do you have any
new questions? What connections did you make between the lab and
lecture?):
Answer
1. Describe your hypothesis.
Only molecules that are smaller than a certain size can pass through a dialysis membrane. Starch molecules would not be able through a dialysis membrane. However, any smaller molecules, such as glucose, would. This experiment illustrates the importance of membranes within a cell. The membrane is essential in allowing certain materials into and out of the cell.
2. What is the point of having negative and positive controls?
Negative control is not expected to have any effect. Positive control is known to have an effect on the results of the experiment.
3. The procedure is set up experimentally
4.Which substance crossed the dialysis membrane
Table 3 shows that the iodine and starch molecules could pass through the dialysis membranes and into the starch solution. The glucose molecules, however, were small enough to get through the dialysis tubing membrane and into water.
5.Which molecular remained in the dialysis bag?
Starch molecules, which are very large polysaccharides were unable to pass through membrane and remained in the bag.
6. Did all the molecules escape from the bag and get into the beaker Why or why not?
All molecules were not able to diffuse from the bag into the beaker. Only glucose and water molecules could escape the bag. While all molecules of a substance are approximately the same size, molecules from different substances have different sizes. The measurements show that water molecules have a very small size, while glucose molecules are much larger and starch molecules very large.
Conclusion
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